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Literature summary extracted from
- A loop of coagulation factor VIIa influencing macromolecular substrate specificity (2007), FEBS Lett., 581, 71-76.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.21 | tissue factor | TF, complex formation with factor VIIa, structure, overview | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.21.21 | E154R | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | E296R | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | K192Q | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | K341Q | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | M156K | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | M156Q | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | M298K | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | M298Q | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | Q143R | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | Q176G | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | Q286R | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | Q40G | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | T151Q | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | T293Q | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | V158E | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
| 3.4.21.21 | V21E | site-directed mutagenesis, activity and active site structure in comparison to the wild-type | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.21 | antithrombin | - |
Homo sapiens |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.21 | Ca2+ | binding structure | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.21 | factor IX + H2O | Homo sapiens | - |
factor IXa + ? | - |
? | |
| 3.4.21.21 | Factor X + H2O | Homo sapiens | - |
Factor Xa + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.21 | Homo sapiens | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.21 | additional information | - |
substrate specificity of wild-type and mutant enzymes | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.21 | benzyloxycarbonyl-D-Arg-Gly-Arg-4-nitroanilide + H2O | chromogenic S-2765 | Homo sapiens | ? | - |
? | |
| 3.4.21.21 | D-Ile-Pro-Arg-4-nitroanilide + H2O | chromogenic substrate S-2288 | Homo sapiens | D-Ile-Pro-Arg + 4-nitroaniline | - |
? | |
| 3.4.21.21 | factor IX + H2O | - |
Homo sapiens | factor IXa + ? | - |
? | |
| 3.4.21.21 | Factor X + H2O | - |
Homo sapiens | Factor Xa + ? | - |
? | |
| 3.4.21.21 | Factor X + H2O | macromolecular substrate interactions at exosites, sites within the FVIIa-TF complex distant from the active site of FVIIa, determine affinity and specificity in the productive recognition of FX | Homo sapiens | Factor Xa + ? | - |
? | |
| 3.4.21.21 | additional information | a loop of factor VIIa influences the macromolecular substrate specificity, overview | Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.21 | FVIIa | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.21 | 22 | - |
assay at room temperature | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.21 | 7.4 | - |
assay at | Homo sapiens |
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